Cell Bank

GLIOMA CELL LINES

Our laboratory conducts experimental research using two human glioblastoma cell lines: M059K and M059J. Both lines are derived from malignant brain tumor cells and are extensively utilized in the fields of radiobiology, molecular genetics, and oncopharmacology. The cell lines were obtained from the American Type Culture Collection (ATCC).

• M059K Cell Line – this line exhibits relative resistance to ionizing radiation, owing to functional DNA damage response (DDR) pathways. M059K cells possess efficient DNA repair mechanisms, particularly for double-strand break (DSB) repair, which renders them less susceptible to radiation-induced genomic damage. These cells serve as a valuable model for investigating cellular radioprotection and stress tolerance mechanisms.
• M059J Cell Line – in contrast, M059J cells demonstrate marked hypersensitivity to ionizing radiation due to the absence of a key protein involved in DSB repair. This deficiency makes M059J an ideal model system for studying DNA damage response pathways and evaluating genome-targeting anticancer therapies, particularly those based on synthetic lethality or radiosensitization strategies.

Working with these complementary cell lines enables comparative analyses of tumor cell responses to genotoxic stress, providing critical insights into heterogeneity in therapy resistance and facilitating the development of more effective, personalized cancer treatments.